Single-channel blind estimation of arterial input function and tissue impulse response in DCE-MRI

Multipass dynamic MRI and pharmacokinetic modeling are used to estimate perfusion parameters of leaky capillaries. Curve fitting and nonblind deconvolution are the established methods to derive the perfusion estimates from the observed arterial input function (AIF) and tissue tracer concentration function. These nonblind methods are sensitive to errors in the AIF, measured in some nearby artery or estimated by multichannel blind deconvolution. Here, a single-channel blind deconvolution algorithm is presented, which only uses a single tissue tracer concentration function to estimate the corresponding AIF and tissue impulse response function. That way, many errors affecting these functions are reduced. The validity of the algorithm is supported by simulations and tests on real data from mouse. The corresponding nonblind and multichannel methods are also presented.     

Layer‐by‐Layer Coated Gold Nanoparticles: Size‐Dependent Delivery of DNA into Cells

Because nanoparticles are finding uses in myriad biomedical applications, including the delivery of nucleic acids, a detailed knowledge of their interaction with the biological system is of utmost importance. Here the size‐dependent uptake of gold nanoparticles (AuNPs) (20, 30, 50 and 80 nm), coated with a layer‐by‐layer approach with nucleic acid and poly(ethylene imine) (PEI), into a variety of mammalian cell lines is studied. In contrast to other studies, the optimal particle diameter for cellular uptake is determined but also the number of therapeutic cargo molecules per cell. It is found that 20 nm AuNPs, with diameters of about 32 nm after the coating process and about 88 nm including the protein corona after incubation in cell culture medium, yield the highest number of nanoparticles and therapeutic DNA molecules per cell. Interestingly, PEI, which is known for its toxicity, can be applied at significantly higher concentrations than its IC₅₀ value, most likely because it is tightly bound to the AuNP surface and/or covered by a protein corona. These results are important for the future design of nanomaterials for the delivery of nucleic acids in two ways. They demonstrate that changes in the nanoparticle size can lead to significant differences in the number of therapeutic molecules delivered per cell, and they reveal that the toxicity of polyelectrolytes can be modulated by an appropriate binding to the nanoparticle surface.     

Antibacterial Surface Coatings from Zinc Oxide Nanoparticles Embedded in Poly(N‐isopropylacrylamide) Hydrogel Surface Layers

Despite multiple research approaches to prevent bacterial colonization on surfaces, device‐associated infections are currently responsible for about 50% of nosocomial infections in Europe and significantly increase health care costs, which demands development of advanced antibacterial surface coatings. Here, novel antimicrobial composite materials incorporating zinc oxide nanoparticles (ZnO NP) into biocompatible poly(N‐isopropylacrylamide) (PNIPAAm) hydrogel layers are prepared by mixing the PNIPAAm prepolymer with ZnO NP, followed by spin‐coating and photocrosslinking. Scanning electron microscopy (SEM) characterization of the composite film morphology reveals a homogeneous distribution of the ZnO NP throughout the film for every applied NP/polymer ratio. The optical properties of the embedded NP are not affected by the matrix as confirmed by UV‐vis spectroscopy. The nanocomposite films exhibit bactericidal behavior towards Escherichia coli (E. coli) for a ZnO concentration as low as ≈0.74 μg cm^?2 (1.33 mmol cm^?3), which is determined by inductively coupled plasma optical emission spectrometry. In contrast, the coatings are found to be non‐cytotoxic towards a mammalian cell line (NIH/3T3) at bactericidal loadings of ZnO over an extended period of seven days. The differential toxicity of the ZnO/hydrogel nanocomposite thin films between bacterial and cellular species qualifies them as promising candidates for novel biomedical device coatings.     

A Spectrophotometric Microtiterplate Assay to Determine the Transphosphatidylation Potential of Phospholipase D

In addition to the hydrolysis of the terminal phosphate ester bond in glycerophospholipids, phospholipases D (PLDs) are able to catalyze the exchange of the polar head group. The biocatalytic potential of PLDs strongly depends on the ratio of the transphosphatidylation to hydrolysis rate which, therefore, is an important criterion in the screening for efficient PLDs from natural sources or combinatorial DNA libraries. Here, we present a fast spectrophotometric assay that allows the determination of the rate of both hydrolysis and transphosphatidylation of PLD-containing solutions including cell extracts in one microtiterplate. The assay is based on the reaction of phosphatidylcholine solubilized in Triton X-100 micelles with ethanolamine and the determination of phosphatidic acid (PA) and choline. PA is determined via Fe(III) complexation and represents hydrolysis, while choline is determined by the conventional choline oxidase/peroxidase assay and yields the total conversion. The difference between both values corresponds to the transphosphatidylation product. The method is suitable for measuring reactions rates as well as product yields after defined time periods. As shown for E. coli cells expressing PLD from cabbage, the assay can be applied to extracts of cells grown and lysed in microtiterplates.     

Sustainability standards for bioenergy—A means to reduce climate change risks?

The paper discusses the importance of standards for sustainable bioenergy production. Sustainability of bioenergy production is crucial if bioenergy is supposed to contribute effectively to climate change mitigation. First, a brief overview of current bioenergy policies and of initiatives and legislation for bioenergy sustainability are given. Then, the authors show that under free market conditions undersupply of sustainable bioenergy will prevail. Two types of market failures are identified: information asymmetry and externalities in bioenergy production. Due to these market failures bioenergy is less sustainable than it could be. It is shown that mandatory certification and subsequent labeling can help to overcome the information asymmetry and lead to a more efficient market outcome since consumers can choose products according to their preferences. The authors conclude, however, that the existence of production externalities asks for stronger market intervention, for example in the form of binding minimum standards or taxes. The paper discusses the efficiency and feasibility of such policy measures and shows that mandatory certification combined with binding minimum standards can be an adequate policy choice to regulate the bioenergy market.     

Phospholipid Hydrolysis and Transphosphatidylation by Phospholipase D from Indian Mustard Seeds in Two-Phase Systems

Phospholipase D (PLD), isolated from Indian mustard seeds and purified to homogeneity, has recently been identified as typical α‐type PLD with a high activity toward phosphatidylcholine (PC) in an aqueous mixed micellar system (Khatoon et al. 2014, Phytochemistry 117, 65–75). In light of biocatalytical application, we have now studied the enzyme in aqueous‐organic two‐phase systems and compared the results with the properties of the enzyme in aqueous mixed micellar systems. n‐Hexane containing 5–10 mol% of 1‐ or 2‐octanol proved to be optimal as an organic solvent in the two‐phase system, whereas anionic detergents such as sodium dodecyl sulfate or sodium deoxycholate were favorable components in the mixed micellar system. The optimum pH value (5.5–5.6) and the optimum Ca²⁺ ion concentration (70 mM) were independent of the reaction system. The head group selectivity in terms of activity toward different phospholipids (PC > phosphatidylethanolamine > phosphatidylglycerol) was similar in both types of reaction systems. Also, the K_M values toward PC were on the same order of magnitude. In contrast, the V_max value in the two‐phase system was 20‐fold lower than in the mixed micellar system. The enzyme was able to catalyze the substitution of choline in PC by ethanolamine, glycerol, and ethylene glycol with high efficiency. l ‐Serine was exchanged for choline with low activity. Myo‐inositol was not an alcohol acceptor, but promoted the hydrolysis of PC.     

How heterogeneous are adolescents’ preferences for natural and semi-natural riverscapes as recreational settings?

Recent research has indicated that the younger generations may be disconnected from nature and even show some dislike for natural conditions. This study addressed adolescents’ preferences for riverscapes with varying fluvial dynamics as recreational settings. A stated choice survey with digitally calibrated river scenarios asked Austrian pupils (N = 281) about their preferences for several physical and social characteristics of a floodplain landscape. Pupils perceived riverscapes with a high water dynamic as best for recreation, while water bodies with low water levels and algae, dry river sites, floods and littered trails were disliked. Heterogeneity among the pupils was found with one smaller segment preferring settings with high water dynamic and low human impact, while the larger one preferred settings with more human impact and low water dynamic. This segment that shows some dislike for natural conditions had less experience with rivers and scored lower on natural river-related attitudes and perceptions. Implications for recreational river planning and aesthetic assessments are derived.